通过使用运动发酵单胞菌中编码葡萄糖转运蛋白和葡萄糖激酶的基因，在大肠杆菌的葡萄糖阴性突变体中重建葡萄糖摄取和磷酸化过程。
Reconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinase.
作者信息
Snoep J L, Arfman N, Yomano L P, Fliege R K, Conway T, Ingram L O
机构信息
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
出版信息
J Bacteriol. 1994 Apr;176(7):2133-5. doi: 10.1128/jb.176.7.2133-2135.1994.
Abstract
Expression of the Zymomonas mobilis glf (glucose facilitator protein) and glk (glucokinase) genes in Escherichia coli ZSC113 (glucose negative) provided a new functional pathway for glucose uptake and phosphorylation. Both genes were essential for the restoration of growth in glucose minimal medium and for acid production on glucose-MacConkey agar plates.
摘要
运动发酵单胞菌的glf（葡萄糖转运蛋白）和glk（葡萄糖激酶）基因在大肠杆菌ZSC113（葡萄糖阴性）中的表达为葡萄糖摄取和磷酸化提供了一条新的功能途径。这两个基因对于在葡萄糖基本培养基中恢复生长以及在葡萄糖-麦康凯琼脂平板上产酸都是必不可少的。
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