Macdonald R L, Barker J L
Neurology. 1978 Apr;28(4):325-30. doi: 10.1212/wnl.28.4.325.
Mammalian spinal neurons grown in tissue culture were used to study the effects of the four convulsants-penicillin, pentylenetetrazol, picrotoxin, and bicuculline-on these neurons' responses to amino acids. Bath application of all four convulsants produced paroxysmal depolarizing events in the neurons; iontophoresis of the four convulsants selectively depressed responses produced by iontophoresis of the putative inhibitory transmitter GABA, and effected this depression without altering either inhibitory responses to beta-alanine or glycine, or excitation mediated by glutamate. These results support the hypothesis that the convulsant activity of these agents comes in part from selective antagonism of GABA-mediated postsynaptic inhibition.
在组织培养中生长的哺乳动物脊髓神经元被用于研究四种惊厥剂——青霉素、戊四氮、印防己毒素和荷包牡丹碱——对这些神经元对氨基酸反应的影响。将所有四种惊厥剂应用于浴液中，可在神经元中产生阵发性去极化事件；四种惊厥剂的离子导入选择性地抑制了由假定的抑制性递质γ-氨基丁酸（GABA）的离子导入所产生的反应，并且在不改变对β-丙氨酸或甘氨酸的抑制反应或由谷氨酸介导的兴奋的情况下实现这种抑制。这些结果支持了这样一种假说，即这些药物的惊厥活性部分源于对GABA介导的突触后抑制的选择性拮抗作用。
