Hyperthermic treatment (43 degrees C) rapidly impedes attachment of fibroblasts to culture substrates.

Hyperthermic (43 degrees C) exposure of Chinese hamster fibroblasts for more than 30 min, either in suspension or as a monolayer, significantly inhibits their ability to attach to the culture substrate. This finding as well as the effect of hyperthermia on membrane proteins and on surface topology are rapidly expressed and differ from the inhibition of colony formation which is a later effect of hyperthermia on reproductive ability.