Biber T U, Curran P F
J Gen Physiol. 1968 May;51(5):606-20. doi: 10.1085/jgp.51.5.606.
Net inward flux of mannitol across toad skin induced by making the outside solution hypertonic with urea has been investigated. No significant relation between net mannitol flux and net Na flux could be detected when both fluxes were measured simultaneously. In addition, the net mannitol flux caused by hypertonic solution was not altered by inhibition of Na transport with ouabain or by replacement of all Na in the bathing solutions by choline. The rate of net mannitol flux was dependent on the magnitude of the urea concentration difference across the skin and the direction of net flux could be reversed by reversing the direction of the urea concentration difference. These observations suggest that the mannitol transfer is the result of a coupling between the flows of urea and mannitol.
通过用尿素使外部溶液变为高渗来诱导甘露醇穿过蟾蜍皮肤的净内向通量，这一过程已得到研究。当同时测量甘露醇通量和钠通量时，未检测到它们之间存在显著关系。此外，高渗溶液引起的甘露醇净通量不会因哇巴因抑制钠转运或用胆碱替代浴液中的所有钠而改变。甘露醇净通量的速率取决于皮肤两侧尿素浓度差的大小，并且通过反转尿素浓度差的方向可以使净通量的方向反转。这些观察结果表明，甘露醇的转运是尿素和甘露醇流动之间耦合的结果。
