菠菜叶绿体的乙酸盐结合作为脂肪酸合成的一个方面
Acetate binding of spinach chloroplasts as a facet of fatty acid synthesis.
作者信息
Devor K A, Mudd J B
出版信息
Plant Physiol. 1968 Jun;43(6):853-8. doi: 10.1104/pp.43.6.853.
Abstract
A particulate fraction of spinach chloroplasts is the major site of binding when either acetate or acetyl-CoA is used as substrate. The acetate is linked covalently, and the binding is inhibited by reagents which react with sulfhydryl groups. The amount of acetate bound is lowered by both citrate and oxaloacetate; however, the binding is not reversed by oxaloacetate. Reversal of binding is also not brought about by the addition of unlabeled acetyl-CoA. If cofactors for fatty acid synthesis and cold acetyl-CoA are added, the binding of labeled acetate is reversed. Acyl carrier protein from E. coli increases the binding of labeled acetate.
摘要
当以乙酸盐或乙酰辅酶A作为底物时，菠菜叶绿体的颗粒部分是结合的主要部位。乙酸盐以共价键连接，并且与巯基反应的试剂会抑制这种结合。柠檬酸盐和草酰乙酸盐都会降低乙酸盐的结合量；然而，草酰乙酸盐不会使结合逆转。添加未标记的乙酰辅酶A也不会导致结合逆转。如果添加脂肪酸合成的辅助因子和冷乙酰辅酶A，标记乙酸盐的结合会逆转。来自大肠杆菌的酰基载体蛋白会增加标记乙酸盐的结合。
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