Evans D J, Richardson S H
J Bacteriol. 1968 Jul;96(1):126-30. doi: 10.1128/jb.96.1.126-130.1968.
The in vitro production of significant amounts of extracellular choleragen and vascular permeability factor (PF) by Vibrio cholerae strain VC-12 (Ogawa) in a basal peptone medium required forced aeration, low incubation temperature, and a low initial pH. Filtrates of alkaline peptone cultures of VC-12 grown at 37 C contained an ion translocase inhibitory activity but neither choleragen nor PF activity, Sterile filtrates of pH 6.5 peptone cultures of VC-12 grown at 29 C contained no ion translocase inhibitory activity but possessed choleragen activity (lethality for the suckling rabbit) and PF activity to the extent that the intradermal inoculation of 0.1 ml of a 1:12,288 dilution of such a filtrate gave rise to a vascular permeability reaction (8 by 8 mm in diameter) in the guinea pig. PF excretion occurred during the late logarithmic phase of growth but did not appear to be the consequence of cell lysis. The PF activities of strains VC-12 and 569B (Inaba) were neutralized to the same extent by anticholeragen antiserum prepared against crude 569B choleragen.
霍乱弧菌VC - 12（小川型）在基础蛋白胨培养基中大量体外产生细胞外霍乱毒素和血管通透因子（PF），需要强制通气、较低的培养温度和较低的初始pH值。在37℃生长的VC - 12碱性蛋白胨培养物滤液含有离子转位酶抑制活性，但既无霍乱毒素活性也无PF活性；在29℃生长的VC - 12 pH 6.5蛋白胨培养物无菌滤液不含离子转位酶抑制活性，但具有霍乱毒素活性（对乳兔的致死性）和PF活性，以至于皮内接种0.1 ml这种滤液的1:12,288稀释液可在豚鼠中引起血管通透性反应（直径8×8 mm）。PF排泄发生在生长对数后期，但似乎不是细胞裂解的结果。针对粗制569B霍乱毒素制备的抗霍乱毒素抗血清对菌株VC - 12和569B（稻叶型）的PF活性中和程度相同。
