Aver'ianov A V, Mikhaĭlov G A, Zerov Iu P, Khovanskikh A E
Mol Biol (Mosk). 1978 May-Jun;12(3):646-53.
The aim of this work was to investigate the interrelationship between RNA biosynthesis and that of protein in chick liver during experimental coccidiosis induced by E. tenella. The peculiarity of this model is that in the course of this disease protein synthesis is significantly intensified inspite of the fact that the rate of the biosynthesis is rather high under normal conditions. It has been shown that 4 to 6 days after infection incorporation of labeled amino acids into proteins from chick liver subcellular fractions is greatly increased. The most pronounced changes are in ribosomal and mitochondrial fractions as well as in the postribosomal supernatant. At the same time the specific radioactivity of serum albumin excreted by liver was increased by factor 3. These changes in protein biosynthesis are associated with a significant increase of both the content and intensity of biosynthesis of high molecular weight precursors of rRNA as well as with those of mature 18S rRNA. The amount of 28S rRNA and mRNA per cell is practically without any changes whereas the mRNA turnover is somewhat more extensive. The selective accumulation of 18S rRNA is suggested to be responsible for the intensification of protein biosynthesis.
本研究旨在探讨柔嫩艾美耳球虫诱导的实验性球虫病期间，雏鸡肝脏中RNA生物合成与蛋白质生物合成之间的相互关系。该模型的独特之处在于，尽管在正常条件下生物合成速率相当高，但在疾病过程中蛋白质合成仍显著增强。研究表明，感染后4至6天，雏鸡肝脏亚细胞组分中标记氨基酸掺入蛋白质的量大幅增加。最显著的变化出现在核糖体和线粒体组分以及核糖体后上清液中。与此同时，肝脏分泌的血清白蛋白的比放射性增加了3倍。蛋白质生物合成的这些变化与rRNA高分子量前体生物合成的含量和强度显著增加以及成熟18S rRNA的生物合成增加有关。每个细胞中28S rRNA和mRNA的量实际上没有任何变化，而mRNA周转则更为广泛。18S rRNA的选择性积累被认为是蛋白质生物合成增强的原因。
