标准接种物的液氮保存：气相储存
Liquid nitrogen preservation of standard inoculum: gas-phase storage.
作者信息
McDaniel L E, Bailey E G
出版信息
Appl Microbiol. 1968 Jun;16(6):912-6. doi: 10.1128/am.16.6.912-916.1968.
Abstract
Liquid nitrogen storage was the most satisfactory of several methods tested for supplying standard Streptomyces viridoflavus inoculum for laboratory and pilot plant experimentation. Shake-flask cultures were subdivided into sterile cotton-plugged ampoules and stored in the gas space of a liquid nitrogen refrigerator. There were no detectable changes in viability or in candidin-producing capacity over a 12-month test period. The procedure also proved satisfactory with all other organisms tested.
摘要
在为实验室和中试工厂实验提供标准绿黄链霉菌接种物而测试的几种方法中，液氮保存是最令人满意的。摇瓶培养物被分装到无菌棉塞安瓿中，并储存在液氮冰箱的气相中。在12个月的测试期内，活力或制假丝菌素能力没有可检测到的变化。该方法对所有其他测试的微生物也证明是令人满意的。
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本文引用的文献
1
2
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3
CRYOBIOLOGY AS VIEWED BY THE MICROBIOLOGIST.
Cryobiology. 1964 Sep-Oct;51:18-39. doi: 10.1016/0011-2240(64)90017-3.
4
LIQUID NITROGEN FREEZING IN MICROBIOLOGICAL ASSAY SYSTEMS. I. PRESERVATION OF LACTOBACILLUS LEICHMANNII FOR DIRECT USE IN THE VITAMIN B12 ASSAY.微生物检测系统中的液氮冷冻。I. 用于维生素B12检测直接使用的莱氏乳杆菌的保存
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