在海德果蝇的多线核中一种位点特异性核糖核蛋白产物的产生与释放。
Production and release of a locus-specific ribonucleoprotein product in polytene nuclei of Drosophila hydei.
作者信息
Derksen J, Berendes H D, Willart E
出版信息
J Cell Biol. 1973 Dec;59(3):661-8. doi: 10.1083/jcb.59.3.661.
Abstract
A specific, 0.1-0.3-microm large ribonucleoprotein complex consisting of a central core with stalklike extensions on top of which 280-320-A ribonucleoprotein particles are situated is found in an experimentally activated chromosome region, 2-48C, of the polytene chromosomes of Drosophila hydei. Alkaline hydrolysis, RNAse digestion, and uranyl-EDTA-lead staining indicated the ribonucleoprotein character of the 280-320-A particles, whereas the central core seems to be devoid of RNA. The characteristic complexes are present in the nucleoplasm and at the nuclear membrane, but absent from the cytoplasm. It is suggested that the large RNP complexes are the specific products of the puff at 2-48C. Complexes similar to the ones described have not been observed in any other region of the polytene salivary gland chromosomes of this species.
摘要
在海德氏果蝇多线染色体实验激活的2 - 48C染色体区域中，发现了一种特定的、大小为0.1 - 0.3微米的核糖核蛋白复合体，它由一个中央核心和顶部的茎状延伸部分组成，在茎状延伸部分之上分布着280 - 320埃的核糖核蛋白颗粒。碱性水解、核糖核酸酶消化以及铀酰 - 乙二胺四乙酸 - 铅染色表明280 - 320埃颗粒具有核糖核蛋白的特性，而中央核心似乎不含RNA。这些特征性复合体存在于核质和核膜处，但细胞质中没有。有人认为，大型核糖核蛋白复合体是2 - 48C处胀泡的特定产物。在该物种多线唾液腺染色体的任何其他区域都未观察到与所描述的复合体类似的结构。
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