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通过体细胞杂交将磷酸丙糖异构酶基因定位到小家鼠的6号染色体上,并将三肽酶-1基因定位到10号染色体上。

Assignment of the genes for triose phosphate isomerase to chromosome 6 and tripeptidase-1 to chromosome 10 in Mus musculus by somatic cell hybridization.

作者信息

Leinwand L A, Kozak C A, Ruddle F H

出版信息

Somatic Cell Genet. 1978 Mar;4(2):233-40. doi: 10.1007/BF01538987.

Abstract

Evidence is presented for the assignment of the gene for triose phosphate isomerase to Mus musculus chromosome 6 and tripeptidase-1 to chromosome 10 by synteny testing and chromosome assignment in Chinese hamster X mouse somatic cell hybrid clones. Neither TPI nor TRIP-1 were expressed concordantly with any known isozyme markers in 45 hybrid clones (13 primary and 32 secondary). Karyotypic analysis of 21 clones showed that the expression of TPI and chromosome 6 were concordant in all cases as was expressed of TRIP-1 and chromosome 10. Both chromosomes were previously unmarked by isozymes.

摘要

通过在中国仓鼠X小鼠体细胞杂交克隆中进行同线测试和染色体定位,提出了磷酸丙糖异构酶基因定位于小家鼠6号染色体以及三肽酶-1定位于10号染色体的证据。在45个杂交克隆(13个原代克隆和32个二代克隆)中,TPI和TRIP-1均未与任何已知的同工酶标记一致表达。对21个克隆的核型分析表明,TPI的表达与6号染色体在所有情况下均一致,TRIP-1的表达与10号染色体也一致。这两条染色体以前都没有同工酶标记。

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