小鼠神经母细胞瘤细胞中毒蕈碱型乙酰胆碱受体的配体结合特性
Ligand-binding propeties of the muscarinic acetylcholine receptor in mouse neuroblastoma cells.
作者信息
Strange P G, Birdsall N J, Burgen A S
出版信息
Biochem J. 1978 Jun 15;172(3):495-501. doi: 10.1042/bj1720495.
Abstract
- Muscarinic acetylcholine receptors in a plasma-membrane fraction derived from mouse neuroblastoma clone NIE-115 bind [3-3H]quinuclidinyl benzilate according to the Law of Mass Action (Kdissociation 40 pM, h0.96). 2. Antagonist and agonist binding to the receptor was studied by displacement of [3-3H]quinuclidinyl benzilate with non-radioactive ligands. The data show good agreement with similar data obtained on rat brain and ideal smooth muscle [Birdsall & Hulme (1976) J. Neurochem. 27, 7-16] indicating that the receptor is very similar in these three tissues.
摘要
- 源自小鼠神经母细胞瘤克隆NIE-115的质膜组分中的毒蕈碱型乙酰胆碱受体根据质量作用定律结合[3-3H]喹核醇基苯甲酸酯（解离常数K为40 pM，h为0.96）。2. 通过用非放射性配体取代[3-3H]喹核醇基苯甲酸酯来研究拮抗剂和激动剂与该受体的结合。数据与在大鼠脑和理想平滑肌上获得的类似数据显示出良好的一致性[伯德索尔和休姆（1976年）《神经化学杂志》27卷，7 - 16页]，表明该受体在这三种组织中非常相似。
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