1,2:5,6-二脱水半乳糖醇对脱氧核糖核酸和鸟苷的烷基化作用。
Alkylation by 1,2:5,6-dianhydrogalactitol of deoxyribonucleic acid and guanosine.
作者信息
Institóris E, Tamás J
出版信息
Biochem J. 1980 Mar 1;185(3):659-66. doi: 10.1042/bj1850659.
DNA was alkylated in neutral solution at 37 degrees C with 1,2:5,6-dianhydrogalactitol and hydrolysed to yield two principal products, identified as 7-galactitylguanine and 1,6-dideoxy-1,6-di(guanin-7-yl)galactitol. The reaction products were separated by chromatography on Sephadex G-10 and Dowex 50 (H+ form). The two compounds were also obtained by reaction between dianhydrogalactitol and guanosine in acetic acid. The products were characterized from their u.v.-spectral data by comparison with those of the 7-alkylguanines and were also identified by mass spectrometry.
在37℃的中性溶液中，用1,2:5,6 - 二脱水半乳糖醇使DNA发生烷基化反应，然后水解生成两种主要产物，经鉴定为7 - 半乳糖基鸟嘌呤和1,6 - 二脱氧 - 1,6 - 二（鸟嘌呤 - 7 - 基）半乳糖醇。反应产物通过在Sephadex G - 10和Dowex 50（H⁺型）上的色谱法进行分离。这两种化合物也可通过二脱水半乳糖醇与鸟苷在乙酸中反应得到。通过与7 - 烷基鸟嘌呤的紫外光谱数据进行比较，从产物的紫外光谱数据对其进行了表征，并且还通过质谱法进行了鉴定。
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