Burnett J P, Summers A O, Harrington J A, Dwyer A C
Appl Microbiol. 1968 Aug;16(8):1245-50. doi: 10.1128/am.16.8.1245-1250.1968.
A method is described for increasing the incorporation of radioactive thymidine into adenovirus deoxyribonucleic acid by the use of amethopterin. In addition, a modified procedure is presented for the preparation of highly purified adenoviruses. This procedure, which employs enzymatic digestion of cellular debris, obviates the necessity for fluorocarbon treatment of crude virus suspensions, and routinely provides excellent recovery of virus.
描述了一种通过使用氨甲蝶呤增加放射性胸苷掺入腺病毒脱氧核糖核酸的方法。此外，还提出了一种改进的方法用于制备高度纯化的腺病毒。该方法采用酶消化细胞碎片，避免了对粗病毒悬液进行氟碳处理的必要性，并且常规可实现病毒的良好回收率。
