Pocock D H
J Gen Virol. 1978 Jul;40(1):93-101. doi: 10.1099/0022-1317-40-1-93.
The effect of sulphydryl reagents on haemagglutinating encephalomyelitis virus (HEV), a coronavirus of pigs, was investigated. Using increasing concentrations of dithiothreitol (DTT), 50% of the virus infectivity and haemagglutination (HA) activity could be removed by i.5 mM and 4 to 5 mM respectively. The effect of DTT concentrations on the polypeptide composition was also examined. Of the three external glycoproteins gp 125 was found to be the most susceptible, 50% being removed by incubation of the virus with 5 to 6 mM-DTT. Of the other two glycoproteins gp 180 was unaffected by DDT concentrations up to 100 mM and the amount of gp 100 gradually declined at concentrations above 20 mM. The rates of removal of the virus HA activity and gp 125 suggested that this polypeptide was an essential part of the virus haemagglutinin. The lack of evidence for any interpeptide disulphide bonds suggested that the loss of these glycoproteins was due to an alteration in their conformation brought about by the cleavage of intrapeptide disulphide bonds. The loss of protein from the surface of the virus resulted in a change in the virus morphology with the appearance of thin fibrous projections instead of the characteristic petal-like coronavirus projections.
研究了巯基试剂对猪冠状病毒血凝性脑脊髓炎病毒（HEV）的影响。使用浓度递增的二硫苏糖醇（DTT），分别在1.5 mM和4至5 mM时可去除50%的病毒感染性和血凝（HA）活性。还检测了DTT浓度对多肽组成的影响。在三种外部糖蛋白中，发现gp 125最敏感，将病毒与5至6 mM - DTT孵育可去除50%。在其他两种糖蛋白中，gp 180在高达100 mM的DDT浓度下不受影响，而gp 100的量在浓度高于20 mM时逐渐下降。病毒HA活性和gp 125的去除速率表明该多肽是病毒血凝素的重要组成部分。缺乏任何肽间二硫键的证据表明这些糖蛋白的丢失是由于肽内二硫键断裂导致其构象改变。病毒表面蛋白质的丢失导致病毒形态发生变化，出现细纤维状突起，而不是特征性的花瓣状冠状病毒突起。
