Mukkur T K
Am J Vet Res. 1978 Aug;39(8):1269-73.
Inoculation of calves with potassium thiocyanate (KSCN) extract of Pasteurella multocida type A in saline-tris buffer or in Freund's incomplete adjuvant or modified Freund's incomplete adjuvant resulted in the elicitation of agglutinating, hemagglutinating, bactericidal and homocytotropic antibodies. The antibody response was significantly (P = 0.05) higher in calves inoculated with the KSCN extract in either adjuvant than in those inoculated with the extract in saline-tris buffer. Hemagglutination also was observed if the KSCN extract of P hemolytica was used for sensitizing the tanned sheep red blood cells. Further, calf anti-P multocida extract antisera also was bactericidal to P hemolytica. The KSCN extract of P multocida was found to be nontoxic to calves at 2 doses tested, as judged by an evaluation of total and differential leukocyte counts, body temperature, and pulse rates at various intervals after inoculation.
用A型多杀性巴氏杆菌的硫氰酸钾（KSCN）提取物在生理盐水 - 三羟甲基氨基甲烷缓冲液中或在弗氏不完全佐剂或改良弗氏不完全佐剂中接种犊牛，可诱发出凝集、血凝、杀菌和嗜同种细胞抗体。接种了含佐剂的KSCN提取物的犊牛的抗体反应显著（P = 0.05）高于接种了生理盐水 - 三羟甲基氨基甲烷缓冲液中提取物的犊牛。如果用溶血巴氏杆菌的KSCN提取物致敏鞣酸处理的绵羊红细胞，也会观察到血凝现象。此外，犊牛抗多杀性巴氏杆菌提取物抗血清对溶血巴氏杆菌也有杀菌作用。通过对接种后不同时间间隔的全血细胞计数、分类计数、体温和脉搏率进行评估判断，发现多杀性巴氏杆菌的KSCN提取物在测试的2个剂量下对犊牛无毒。
