Leslie S W, Gad S C, Acosta D
Toxicology. 1978 Jul;10(3):281-9. doi: 10.1016/0300-483x(78)90078-1.
Butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) at concentrations of 100 ppm (0.45 and 0.55 mM, respectively) produced a marked leakage of lactic dehydrogenase (LDH) from cultured myocardial and endotheloid cells into the culture medium. At this concentration both BHT and BHA markedly depressed the beating rate of cultured heart cells with maximum inhibition occurring within 1 h after antioxidant exposure. Morphologically the appearance of cells in the presence of 100 ppm BHT and BHA was similar to the appearance of control cells. However, when BHT and BHA concentrations were increased to 1000 ppm (4.5 and 5.5 mM, respectively), marked cell lysis was seen after a 1 h exposure period. The results of this study suggest that both BHT and BHA, in relatively large concentrations, produce injury to myocardial cells in culture.
浓度为100 ppm（分别为0.45和0.55 mM）的丁基羟基甲苯（BHT）和丁基羟基茴香醚（BHA）可导致培养的心肌细胞和内皮样细胞中的乳酸脱氢酶（LDH）显著渗漏到培养基中。在此浓度下，BHT和BHA均显著降低培养的心脏细胞的搏动率，抗氧化剂暴露后1小时内抑制作用达到最大。形态学上，存在100 ppm BHT和BHA时细胞的外观与对照细胞的外观相似。然而，当BHT和BHA浓度增加到1000 ppm（分别为4.5和5.5 mM）时，暴露1小时后可见明显的细胞裂解。本研究结果表明，相对高浓度的BHT和BHA均可对培养的心肌细胞造成损伤。
