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A continuous-flow culture system for organ culture of large explants of adult tissue: effect of oxygen tension on mouse molar periodontium.

作者信息

Yen E H, Melcher A H

出版信息

In Vitro. 1978 Oct;14(10):811-8. doi: 10.1007/BF02616150.

DOI:10.1007/BF02616150
PMID:569117
Abstract

A modified continuous-flow culture system (CFCS) was developed to maintain large explants of periodontium from adult mouse in organ culture. The culture medium was stored in a reservoir outside of the incubator, pumped via polyvinyl tubing into small glass culture chambers that were placed in the oxygenator and then collected in a waste flask. Medium was analyzed for pO2, pCO2 and pH during the culture period. Three-molar and single-molar explants of periodontium were maintained for 48 hr in the CFCS at two different pO2 ranges: 100 to 120 mm Hg and 400 to 420 mm Hg. [3H]Proline was added 24 hr prior to sacrifice. Light-microscope morphological and radioautographic observations suggested that cell viability and incorporation of [3H]proline, probably into newly synthesized protein, increased with an increase in pO2 and was related to a pO2 gradient extending from the periphery to the center of the explants.

摘要

相似文献

1
A continuous-flow culture system for organ culture of large explants of adult tissue: effect of oxygen tension on mouse molar periodontium.
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2
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Effect of ascorbic acid on protein synthesis and collagen hydroxylation in continuous flow organ cultures of adult mouse periodontal tissues.抗坏血酸对成年小鼠牙周组织连续流动器官培养中蛋白质合成和胶原羟化的影响。
Calcif Tissue Int. 1982 Jul;34(4):408-15. doi: 10.1007/BF02411276.
3
The effect of oxygen partial pressure on protein synthesis and collagen hydroxylation by mature periodontal tissues maintained in organ cultures.

本文引用的文献

1
CORRELATION OF BONE RESORPTION AND FORMATION WITH THE PHYSICAL BEHAVIOR OF LOADED BONE.骨吸收与形成与负荷骨物理行为的相关性。
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Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).NCTC克隆929（L株）小鼠细胞亚系在一种简单的化学成分确定培养基（MB 752/1）中的快速增殖。
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The culture of mature organs in a synthetic medium.在合成培养基中对成熟器官进行培养。
氧分压对器官培养中成熟牙周组织蛋白质合成及胶原羟化作用的影响。
Biochem J. 1979 Mar 15;178(3):605-12. doi: 10.1042/bj1780605.
Exp Cell Res. 1959 Jan;16(1):118-47. doi: 10.1016/0014-4827(59)90201-0.
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Effect of ascorbic acid on protein synthesis and collagen hydroxylation in continuous flow organ cultures of adult mouse periodontal tissues.抗坏血酸对成年小鼠牙周组织连续流动器官培养中蛋白质合成和胶原羟化的影响。
Calcif Tissue Int. 1982 Jul;34(4):408-15. doi: 10.1007/BF02411276.
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Organ culture under hyperbaric oxygen.高压氧下的器官培养
Exp Cell Res. 1967 Feb;45(2):385-98. doi: 10.1016/0014-4827(67)90188-7.
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The effect of various oxygen tensions on the synthesis and degradation of bone collagen in tissue culture.不同氧张力对组织培养中骨胶原合成与降解的影响。
Proc Soc Exp Biol Med. 1966 Mar;121(3):869-72. doi: 10.3181/00379727-121-30911.
7
Studies on hypoxia. II. Autoradiographic quantitation of proline-3H incorporation by connective tissue cells of the neonatal hamster.缺氧研究。II. 新生仓鼠结缔组织细胞对脯氨酸-3H掺入的放射自显影定量分析
J Dent Res. 1968 Mar-Apr;47(2):244-51. doi: 10.1177/00220345680470020901.
8
Changes in vasculature of the periodontium associated with tooth movement in the rhesus monkey and dog.恒河猴和狗牙齿移动过程中牙周组织血管系统的变化。
Arch Oral Biol. 1970 Dec;15(12):1125-32. doi: 10.1016/0003-9969(70)90003-8.
9
In vivo bioelectric petentials in the dentoalveolar complex.牙槽复合体中的体内生物电电位。
Am J Orthod. 1974 Aug;66(2):130-9. doi: 10.1016/0002-9416(74)90232-2.
10
Method fof maintaining large explants or mature mixed tissue in organ culture: molar tooth and supporting structures from the mouse.在器官培养中维持大型外植体或成熟混合组织的方法：来自小鼠的磨牙及支持结构。 （注：原文中“fof”应改为“for”）
In Vitro. 1974 Jul-Aug;10:6-11. doi: 10.1007/BF02615332.