Sodek J, Feng J, Yen E H, Melcher A H
Calcif Tissue Int. 1982 Jul;34(4):408-15. doi: 10.1007/BF02411276.
A continuous flow organ culture system (CFCS) was used to determine the effect of ascorbic acid on the synthesis of collagen and noncollagenous protein by bone of the alveolar process and periodontal ligament in organ cultures of adult mouse periodontium. For the last 24 h of 2 day cultures, 5 microCi/ml 3H-proline was added to the medium. Highly purified collagenase was used to separate the collagenous and noncollagenous proteins and the incorporation of isotope into each fraction measured. Collage synthesized in the presence of less than 10 micrograms/ml ascorbic acid was found to be highly under-hydroxylated (pro:hypro ap. acts. 2.3-3.1) in both tissues. When the ascorbic acid levels were between 25 and 100 micrograms/ml, the synthesis of collagenous proteins was selectively stimulated and hydroxylation significantly improved (pro:hypro sp. acts 1.72-1.89). The effect of ascorbic acid was not related to tissue viability since tissues cultured initially in the absence of ascorbic acid were able to recover completely when compared to controls given ascorbic acid continuously. The proportion of radioactivity in collagen and noncollagenous protein, collagen hydroxylation, and percentage of collagen synthesized as type III (av.23%) in bone of the alveolar process was similar to that found in vivo. However, in the periodontal ligament in vitro the proportion of noncollagenous protein synthesized was increased from 70% to 87% and the percentage of type III collagen increased from 14% to 26% compared to in vivo results.
采用连续流动器官培养系统（CFCS），以确定抗坏血酸对成年小鼠牙周组织器官培养中牙槽突骨和牙周韧带胶原蛋白及非胶原蛋白合成的影响。在为期2天的培养的最后24小时，向培养基中添加5微居里/毫升的3H-脯氨酸。使用高纯度胶原酶分离胶原蛋白和非胶原蛋白，并测定各组分中同位素的掺入量。发现在抗坏血酸浓度低于10微克/毫升的情况下，两种组织中合成的胶原蛋白高度羟基化不足（脯氨酸：羟脯氨酸活性比为2.3 - 3.1）。当抗坏血酸水平在25至100微克/毫升之间时，胶原蛋白的合成受到选择性刺激，羟基化显著改善（脯氨酸：羟脯氨酸比活性为1.72 - 1.89）。抗坏血酸的作用与组织活力无关，因为与持续给予抗坏血酸的对照组相比，最初在无抗坏血酸条件下培养的组织能够完全恢复。牙槽突骨中胶原蛋白和非胶原蛋白的放射性比例、胶原蛋白羟基化以及III型胶原蛋白合成百分比（平均23%）与体内情况相似。然而，与体内结果相比，体外培养的牙周韧带中合成的非胶原蛋白比例从70%增加到87%，III型胶原蛋白百分比从14%增加到26%。
