Carlsson J
In Vitro. 1978 Oct;14(10):860-7. doi: 10.1007/BF02616156.
The growth of human glioma cells, cultured as spherical colonies in agarose gel, stopped after about 10 days for both large and small colonies apparently due to an increased osmolality in the gel. When osmolality was kept under control by addition of distilled water, growth continued. However, a continuous increase in the population-doubling period, similar for both large and small colonies, then was observed. The increase persisted although excess amounts of nutrition were added. When the cells were cultured in liquid suspension above a thin layer of agarose gel and most of the medium was repeatedly changed, the colonies continued to grow beyond the limits in gel cultured. HeLa and hamster embryonic lung cell colonies showed a growth pattern in agarose gel similar to the glioma cells. The results imply that the osmolality must be kept under precise control to prevent growth inhibition. However, it seems difficult to ascertain optimal growth in gel culture for more than about 2 weeks probably because of the accumulation of toxic products.
人类胶质瘤细胞在琼脂糖凝胶中培养成球形集落，大约10天后，无论大集落还是小集落的生长均停止，这显然是由于凝胶中渗透压升高所致。当通过添加蒸馏水控制渗透压时，生长继续。然而，随后观察到大小集落的群体倍增时间均持续增加。尽管添加了过量营养物质，这种增加仍持续存在。当细胞在琼脂糖凝胶薄层上方的液体悬浮液中培养且大部分培养基被反复更换时，集落继续生长超过在凝胶培养中的极限。HeLa细胞和仓鼠胚胎肺细胞集落在琼脂糖凝胶中的生长模式与胶质瘤细胞相似。结果表明，必须精确控制渗透压以防止生长抑制。然而，由于有毒产物的积累，似乎难以确定凝胶培养中超过约2周的最佳生长情况。
