Continuous perfusion of mammalian cells embedded in agarose gel threads.
作者信息
Foxall D L, Cohen J S, Mitchell J B
出版信息
Exp Cell Res. 1984 Oct;154(2):521-9. doi: 10.1016/0014-4827(84)90176-9.
PMID:6479241
Abstract
A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.
摘要
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