对包埋在琼脂糖凝胶丝中的哺乳动物细胞进行连续灌注。
Continuous perfusion of mammalian cells embedded in agarose gel threads.
作者信息
Foxall D L, Cohen J S, Mitchell J B
出版信息
Exp Cell Res. 1984 Oct;154(2):521-9. doi: 10.1016/0014-4827(84)90176-9.
PMID:6479241
Abstract
A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.
摘要
本文描述并表征了一种通过将细胞包埋在精细的琼脂糖凝胶丝中来灌注细胞的方法。通过31P核磁共振光谱法测定代谢物进入凝胶丝的扩散速率。结果表明，这种灌注方法能够使中国仓鼠肺成纤维细胞（CHLF）在数小时内保持代谢活跃状态，并具有高水平的细胞内ATP。
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