使用L5178Y小鼠淋巴瘤细胞的胸苷激酶基因座对某些染发剂成分进行致突变试验。
The mutagenic assay of some hair dye components, using the thymidine kinase locus of L5178Y mouse lymphoma cells.
作者信息
Palmer K A, Denunzio A, Green S
出版信息
J Environ Pathol Toxicol. 1978 Sep-Oct;1(1):87-91.
Five hair dye components were tested for their mutagenicity at the TK +/- locus of L5178Y cells. Three of the components, m-phenylenediamine, 2 nitro-p-phenylenediamine, and 4-nitro-o-phenylenediamine, gave a positive response that was dose related; 2,4-diaminoanisole gave a questionable response; and 2,5-diaminoanisole gave a negative response. The assay was carried out in vitro without metabolic activation during a 24-hr chemical exposure period. It is concluded that further in vivo tests are necessary to establish the safety of the hair dyes.
对五种染发剂成分进行了测试，以检测它们在L5178Y细胞的TK+/-位点的致突变性。其中三种成分，间苯二胺、2-硝基对苯二胺和4-硝基邻苯二胺，给出了与剂量相关的阳性反应；2,4-二氨基苯甲醚给出了可疑反应；2,5-二氨基苯甲醚给出了阴性反应。该检测在体外进行，在24小时的化学暴露期内无代谢活化。得出的结论是，有必要进行进一步的体内试验以确定染发剂的安全性。
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