Mayer V W, Goin C J
Mutat Res. 1980 Jul;78(3):243-52. doi: 10.1016/0165-1218(80)90105-6.
A number of procedures were used to test for the potential of 5 hair-dye chemicals, 4-nitro-o-phenylenediamine, 2-nitro-p-phenylenediamine, m-phenylenediamine 2,4-diaminoanisole sulfate and 2,5-diaminoanisole sulfate, to induce genetic damage in yeast strains D3 and D4 of Saccharomyces cerevisiae. Various plate-test procedures, short-term suspension assays in phosphate buffer and suspension assays with liver enzyme activation all proved to be ineffective for demonstrating genetic effects of these chemicals. Only suspension assays in which the yeast cells were treated with the test chemical under growing conditions for up to 72 h were effective in demonstrating the genetic activity of 4-nitro-o-phenylenediamine and 2,4-diaminoanisole sulfate. The implications of these results for testing of mutagens in yeast systems are discussed along with other supportive evidence from the literature.
采用了多种程序来测试5种染发剂化学物质，即4-硝基邻苯二胺、2-硝基对苯二胺、间苯二胺、2,4-二氨基苯甲醚硫酸盐和2,5-二氨基苯甲醚硫酸盐，在酿酒酵母D3和D4菌株中诱导遗传损伤的可能性。各种平板测试程序、在磷酸盐缓冲液中的短期悬浮试验以及有肝酶激活的悬浮试验，都证明无法有效证明这些化学物质的遗传效应。只有在生长条件下用测试化学品处理酵母细胞长达72小时的悬浮试验，才能有效证明4-硝基邻苯二胺和2,4-二氨基苯甲醚硫酸盐的遗传活性。文中讨论了这些结果对酵母系统中诱变剂测试的意义，并结合文献中的其他支持性证据进行了阐述。
