Smith D B, Flavell R B
Biochim Biophys Acta. 1977 Jan 3;474(1):82-97. doi: 10.1016/0005-2787(77)90216-7.
Nucleotide sequence organisation in the genome of rye, Secale cereale, has been studied using renaturation kinetics and S1 nuclease digestion of the renatured products. Approximately 25 to 30% of the genome consists of very slowly renaturing, possibly single copy sequences. Most, if not all, of these sequences are interspersed between repeated sequences and are between 400 and 3500 nucleotide pairs long. Approximately 70 to 75% of the genome consists of repeated sequences. The more highly diverged repeated sequences (30% of the genome) which fail to renature under highly stringent renaturation conditions range from 500 to more than 5000 base pairs long and are interspersed in the chromosomes with less diverged repeated sequences (40% of the genome) which are usually less than 800 nucleotide pairs long. Four percent of the genome consists of very rapidly reannealing sequences which may be inverted duplications. These sequences may be in clusters distributed through at least 30% of the genome.
利用复性动力学和对复性产物的S1核酸酶消化，对黑麦（Secale cereale）基因组中的核苷酸序列组织进行了研究。基因组中约25%至30%由复性非常缓慢的序列组成，可能是单拷贝序列。这些序列中的大多数（如果不是全部的话）散布在重复序列之间，长度在400至3500个核苷酸对之间。基因组中约70%至75%由重复序列组成。在高度严格的复性条件下不能复性的高度分化的重复序列（占基因组的30%）长度从500到超过5000个碱基对不等，它们散布在染色体中，与分化程度较低的重复序列（占基因组的40%）相间分布，后者通常长度小于800个核苷酸对。基因组的4%由复性非常快的序列组成，这些序列可能是反向重复序列。这些序列可能成簇分布，至少占基因组的30%。
