Baker M A, Falk R E, Falk J, Greaves M F
Br J Haematol. 1976 Jan;32(1):13-19. doi: 10.1111/j.1365-2141.1976.tb01870.x.
Heteroantisera with specificity for human monocyte and macrophage antigens (HuMa) have been tested for activity against peripheral blood cells from patients with acute leukaemia. Using indirect immunofluorescence, blast cells from 25/27 patients with acute myelomonocytic leukaemia were reactive with anti-HuMa antisera, whereas blasts from 19/20 patients with acute myeloblastic leukaemia and 13/13 patients with acute lymphoblastic leukaemia were not reactive. The F(ab1)2 fragment of the antibody retained specific activity. Activity of whole serum against myelomonocytic blast cells was specifically removed by absorption with macrophages. These observations indicate that circulating blast cells from patients with acute myelomonocytic leukaemia may carry differentiation antigens characteristic of monocytes and macrophages.
已对具有针对人单核细胞和巨噬细胞抗原（HuMa）特异性的异种抗血清进行了测试，以检测其对急性白血病患者外周血细胞的活性。使用间接免疫荧光法，25/27例急性粒单核细胞白血病患者的原始细胞与抗HuMa抗血清发生反应，而19/20例急性髓细胞白血病患者和13/13例急性淋巴细胞白血病患者的原始细胞无反应。抗体的F(ab1)2片段保留了特异性活性。全血清对粒单核细胞原始细胞的活性可通过巨噬细胞吸附而特异性去除。这些观察结果表明，急性粒单核细胞白血病患者循环中的原始细胞可能携带单核细胞和巨噬细胞特有的分化抗原。
