Davey F R, Cordell J L, Erber W N, Pulford K A, Gatter K C, Mason D Y
Department of Pathology, SUNY Health Science Center, Syracuse.
J Clin Pathol. 1988 Jul;41(7):753-8. doi: 10.1136/jcp.41.7.753.
A new monoclonal antibody, Y1/82A, was raised against phytohaemagglutinin activated peripheral blood mononuclear cells. Using an immunohistochemical technique it was shown that Y1/82A reacts against peripheral blood and bone marrow monocytes and resident macrophages from essentially all human tissues. Y1/82A bound to determinants present in leukaemic cells from patients with acute myelomonocytic leukaemia and acute monocytic leukaemia, but not to neoplastic cells from patients with malignant lymphoproliferative disorders or malignant epithelial tumours. Y1/82A failed to react with other cell types, with the exception of osteoclasts and megakaryocytes. Analysis by Western blotting showed that the antigen detected by antibody Y1/82A was associated with intracellular granules in macrophages. Monoclonal antibody Y1/82A may be useful in the diagnosis of monocytic leukaemias and histiocytic neoplasms and in the identification of macrophages in tissues from various inflammatory and neoplastic conditions.
一种新的单克隆抗体Y1/82A是针对植物血凝素激活的外周血单核细胞产生的。使用免疫组织化学技术表明，Y1/82A与外周血、骨髓单核细胞以及基本上所有人体组织中的常驻巨噬细胞发生反应。Y1/82A与急性粒单核细胞白血病和急性单核细胞白血病患者的白血病细胞中存在的决定簇结合，但不与恶性淋巴增殖性疾病或恶性上皮肿瘤患者的肿瘤细胞结合。除破骨细胞和巨核细胞外，Y1/82A与其他细胞类型不发生反应。蛋白质印迹分析表明，抗体Y1/82A检测到的抗原与巨噬细胞内的颗粒有关。单克隆抗体Y1/82A可能有助于单核细胞白血病和组织细胞肿瘤的诊断，以及在各种炎症和肿瘤情况下识别组织中的巨噬细胞。
