Kabisch H, Arndt R, Thiele H G, Winkler K, Landbeck G
Clin Exp Immunol. 1978 Jun;32(3):399-404.
A heteroantiserum against common human acute lymphocytic leukaemia (ALL) cells was raised in rabbits. This antiserum was rendered specific for common ALL cells by extensive absorption with different human tissue. A specific antigen associated to common ALL cells was solubilized by sodium deoxycholate from ALL plasma membranes and identified by indirect immunofluorescence. By analytical chromatography, two antigenically active peaks were found. The apparent molecular weights were calculated to be 55,000 and 110,000.
用兔制备了一种针对人类常见急性淋巴细胞白血病(ALL)细胞的异种抗血清。通过用不同人体组织进行广泛吸收,使该抗血清对常见ALL细胞具有特异性。用脱氧胆酸钠从ALL细胞膜中溶解出与常见ALL细胞相关的一种特异性抗原,并通过间接免疫荧光进行鉴定。通过分析色谱法,发现了两个具有抗原活性的峰。计算出的表观分子量分别为55,000和110,000。