Tamura N, Shimada A, Chang S
Immunology. 1972 Jan;22(1):131-40.
The reported observations on the slow, low-grade lysis of EAC1-8 were re-examined by using antisera to guinea-pig C9 as a specific inhibitor against C9 to eliminate the possibility that C8 preparations might contain C9 which lyses EAC1-8. Pretreatment of C8 preparation with IgG fraction of the anti-C9 did not result in reduction of the degree of lysis of EAC1-7 by C8. The dose response curve in titration of C8 with EAC1-7 in the absence of C9 was sigmoidal and completely different from that in the presence of excess C9, suggesting that the cell membrane damage caused by C8 might not be the same as that by C9. Sensitized nucleated cells were shown to be lysed similarly by the first eight components of complement, namely C1 to C8.
通过使用抗豚鼠C9血清作为针对C9的特异性抑制剂，重新检查了关于EAC1-8缓慢、低水平溶解的报道观察结果，以消除C8制剂可能含有裂解EAC1-8的C9的可能性。用抗C9的IgG组分预处理C8制剂并未导致C8对EAC1-7的溶解程度降低。在不存在C9的情况下，用EAC1-7滴定C8时的剂量反应曲线呈S形，与存在过量C9时完全不同，这表明C8引起的细胞膜损伤可能与C9引起的不同。致敏有核细胞被补体的前八个成分，即C1至C8，以类似的方式裂解。
