豚鼠补体的第九成分。作为一种α2球蛋白的分离与鉴定。

Immunology. 1971 Mar;20(3):415-25.

A method was described for isolation of the ninth component of guinea-pig complement (C9). Injection of the isolated C9 into rabbits resulted in the production of antisera which contained antibody to a serum protein with an electrophoretic mobility of an α-globulin. The identity of C9 with the α-globulin was proved by means of immunolysoelectrophoresis and quantitative precipitin reaction. The development of the haemolytic band, observed in the α-region by overlaying blood agar containing EAC14235678 after immunoelectrophoresis of guinea-pig serum or purified C9, was completely inhibited by the precipitin line formed with IgG fraction of the antisera. Further, upon incubation of the IgG fraction with varying amounts of guinea-pig serum in the presence of ethylene-diaminetetraacetate, more than 99.5 per cent of C9 activity in serum was precipitated in the antibody excess region and the equivalence zone. It was found only in the antigen excess region and all the other eight components remained unaffected in the supernatant fluids.

本文描述了一种分离豚鼠补体第九成分（C9）的方法。将分离出的C9注射到兔子体内，可产生抗血清，该抗血清含有针对一种电泳迁移率为α球蛋白的血清蛋白的抗体。通过免疫溶菌电泳和定量沉淀反应证明了C9与α球蛋白的一致性。在豚鼠血清或纯化的C9进行免疫电泳后，通过覆盖含有EAC14235678的血琼脂在α区域观察到溶血带的出现，该溶血带被抗血清IgG组分形成的沉淀线完全抑制。此外，在乙二胺四乙酸存在下，将IgG组分与不同量的豚鼠血清一起孵育时，血清中超过99.5%的C9活性在抗体过量区和等价区沉淀。仅在抗原过量区发现沉淀，上清液中的所有其他八个成分均未受影响。