Pennock C A, Passant L P, Bolton F G
J Clin Pathol. 1968 Jul;21(4):518-20. doi: 10.1136/jcp.21.4.518.
Three turbidometric methods and one method using ultraviolet spectrophotometry for estimating total cerebrospinal fluid protein have been examined. The necessity for preliminary dialysis renders the ultraviolet method unsuitable for routine use. The turbidometric method of Meulemans (1960) using a sulphosalicylic acid-sodium sulphate precipitating fluid is better than a method using sulphosalicylic acid alone which is affected by the albumin-globulin ratio, and has a greater sensitivity and better reproducibility than a method using trichloracetic acid as a precipitant. Turbidity may be measured with a spectrophotometer or an MRC grey wedge photometer with human or bovine albumin as a standard. This method deserves wider acceptance.
已对三种比浊法和一种使用紫外分光光度法估算脑脊液总蛋白的方法进行了检验。由于需要进行预透析，紫外法不适用于常规使用。Meulemans（1960年）使用磺基水杨酸 - 硫酸钠沉淀液的比浊法比单独使用磺基水杨酸的方法更好，后者受白蛋白 - 球蛋白比例的影响，并且比使用三氯乙酸作为沉淀剂的方法具有更高的灵敏度和更好的重现性。可以使用分光光度计或MRC灰色楔形光度计，以人或牛白蛋白作为标准来测量浊度。这种方法值得更广泛地采用。
