Ghose T K, Sahai V
Biotechnol Bioeng. 1979 Feb;21(2):283-96. doi: 10.1002/bit.260210213.
The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle ratio are determined as 0.025 hr-1 and 1.2, respectively, for the process. The system is analyzed and steady-state equations for predicting enzyme protein concentrations in the fermentor are developed. In fed-batch cultures, slow addition of cellulose at high concentrations can improve enzyme productivity by as much as 33% over a batch process. The scope and results of using modified medium for cellulase production are also presented.
在实验室规模的发酵罐中研究了提高纤维素发酵过程中酶产量的范围。在使用含有0.5%纤维素的改良培养基进行细胞循环的连续培养过程中，可达到最高产量（30 IU/升·小时）。该过程的最佳稀释率和循环比分别确定为0.025小时⁻¹和1.2。对该系统进行了分析，并建立了用于预测发酵罐中酶蛋白浓度的稳态方程。在分批补料培养中，高浓度下缓慢添加纤维素可使酶产量比分批培养过程提高多达33%。还介绍了使用改良培养基生产纤维素酶的范围和结果。
