Stein-Streilein J, Gross G N, Hart D A
Infect Immun. 1979 Apr;24(1):145-50. doi: 10.1128/iai.24.1.145-150.1979.
The interdependence of the local and systemic immune systems in the development of the immune responses relating to the lung was evaluated. Hamsters were inoculated with 10(9) sheep erythrocytes (SRBC) via local (intratracheal) or systemic (intravenous) routes of immunization. The local immune response was quantitated by the specific antibody-forming cell (sAFC) response in the pulmonary draining lymph nodes (pdLNC). sAFC in the spleens and serum hemagglutination titers evaluated the systemic immune response. The local inoculation of antigen was superior for induction of the maximal numbers of immunoglobulin M (IgM) and non-IgM sAFC in the pdLNC at 4 days post-immunization. However, the local response coould be enhanced by a concomitant splenic response. The local route of inoculation did not consistently induce an sAFC response in the spleen, but when the spleen was involved, two unique observations were recorded regarding the IgM response: (i) an approximate twofold increase in the numbers of sAFC was seen in the pdLNC on day 7 post-inoculation in animals that had a splenic sAFC response; and (ii) the appearance of an early (day 4) serum IgM hemagglutination titer was observed in the animals with a splenic sAFC response. However, the appearance of IgG serum hemagglutination titers was independent of the indirect sAFC response in the spleen but correlated with the appearance of indirect sAFC response in the pdLNC. As expected, intravenous inoculation of antigen was superior for inducing a systemic IgM response. We concluded that the local and systemic immune responses are related and interdependent in providing immune reactivity related to the lung. Whereas the systemic response enhanced both the serum IgM titer and the specific IgM antibody-forming cell response in the draining nodes, we observed that the local response was sufficient for the IgG serum hemagglutination titer even in the absence of a splenic sAFC response.
评估了局部和全身免疫系统在与肺部相关的免疫反应发展中的相互依存关系。通过局部（气管内）或全身（静脉内）免疫途径给仓鼠接种10⁹个绵羊红细胞（SRBC）。通过肺引流淋巴结（pdLNC）中的特异性抗体形成细胞（sAFC）反应来定量局部免疫反应。脾脏中的sAFC和血清血凝滴度评估全身免疫反应。在免疫后4天，局部接种抗原在诱导pdLNC中最大数量的免疫球蛋白M（IgM）和非IgM sAFC方面更具优势。然而，伴随的脾脏反应可增强局部反应。局部接种途径并未始终如一地在脾脏中诱导出sAFC反应，但当脾脏参与时，关于IgM反应记录到两个独特的观察结果：（i）在有脾脏sAFC反应的动物中，接种后第7天pdLNC中sAFC数量大约增加了两倍；（ii）在有脾脏sAFC反应的动物中观察到早期（第4天）血清IgM血凝滴度的出现。然而，IgG血清血凝滴度的出现与脾脏中间接sAFC反应无关，但与pdLNC中间接sAFC反应的出现相关。正如预期的那样，静脉内接种抗原在诱导全身IgM反应方面更具优势。我们得出结论，局部和全身免疫反应在提供与肺部相关的免疫反应性方面是相关且相互依存的。虽然全身反应增强了血清IgM滴度和引流淋巴结中特异性IgM抗体形成细胞反应，但我们观察到即使在没有脾脏sAFC反应的情况下，局部反应对于IgG血清血凝滴度也是足够的。
