Stein-Streilein J, Frazier J L, Gross G N, Hart D A
Infect Immun. 1979 Apr;24(1):139-44. doi: 10.1128/iai.24.1.139-144.1979.
The role of the spleen in the development of specific antibody-forming cells (sAFC) in the pulmonary draining lymph nodes (pdLNC) of hamsters after local inoculation of sheep erythrocytes (SRBC) was evaluated. The role of the spleen was viewed from two vantage points. Panels of animals were either splenectomized with appropriate sham-operated controls before intratracheal inoculation of SRBC, or panels were immunized intravenously simultaneously with the local inoculation of antigen. The presence of an intact spleen was not necessary for the induction of a sAFC response to occur in the pdLNC. Similar numbers of immunoglobulin M (IgM) sAFC were recorded in the pdLNC on day 4 of both sham-operated and splenectomized animals. However, an enhancement of this local response occurred on day 7 if the animals were systemically immunized and therefore demonstrated active participation of the spleen in the specific immune response. The results support the hypothesis that although a local response may occur in the pdLFC in the absence of a spleen or a splenic response, the presence of a systemic or splenic response appears to be important for the enhancement of local IgM sAFC response. These observations suggest that the immune defenses involved in the lower respiratory tract may differ from those in upper respiratory tract and other mucosally lined organs in that the response of the spleen to the antigen affects the local response to that antigen.
评估了在仓鼠经气管内接种绵羊红细胞（SRBC）后，脾脏在肺引流淋巴结（pdLNC）中特异性抗体形成细胞（sAFC）发育中的作用。从两个有利角度审视脾脏的作用。一组动物在经气管内接种SRBC之前进行脾切除，并设置适当的假手术对照，另一组动物在局部接种抗原的同时进行静脉免疫。在pdLNC中诱导sAFC反应并不需要完整脾脏的存在。在假手术组和脾切除组动物的第4天，pdLNC中记录到的免疫球蛋白M（IgM）sAFC数量相似。然而，如果动物进行了全身免疫，在第7天这种局部反应会增强，因此表明脾脏积极参与了特异性免疫反应。结果支持了以下假设：尽管在没有脾脏或脾脏反应的情况下，pdLFC中可能会出现局部反应，但全身或脾脏反应的存在似乎对增强局部IgM sAFC反应很重要。这些观察结果表明，下呼吸道涉及的免疫防御可能与上呼吸道和其他有黏膜衬里的器官不同，因为脾脏对抗原的反应会影响对该抗原的局部反应。
