Masters W H, Johnson V E, Kolodny R C, Tullman G D
Fertil Steril. 1979 Aug;32(2):161-5. doi: 10.1016/s0015-0282(16)44173-7.
Sixteen women ages 20 to 41 participated in a project to evaluate the in vivo intravaginal spermicidal effectiveness of nonoxynol-9 foaming suppositories. Testing of each subject was done at 18 different time intervals ranging from 5 seconds to 6 hours following insertion of the vaginal contraceptive. Simulated coitus using a latex dildo was incorporated in the test design to simulate dispersal of the spermicide that occurs during coitus. Semen specimens meeting uniform criteria for sperm count, motility, morphology, and volume were injected intravaginally; three vaginal aspirates were then immediately obtained to evaluate sperm motility microscopically. Maximal spermicidal effectiveness was found between 5 minutes and 1 hour after insertion of the vaginal contraceptive.
16名年龄在20至41岁之间的女性参与了一项评估壬苯醇醚-9泡沫栓剂体内阴道杀精效果的项目。在插入阴道避孕药后，对每位受试者在从5秒到6小时的18个不同时间间隔进行测试。测试设计中采用乳胶假阴茎模拟性交，以模拟性交过程中杀精剂的扩散。将符合精子计数、活力、形态和体积统一标准的精液标本经阴道注入；然后立即获取三次阴道吸出物，以显微镜评估精子活力。在插入阴道避孕药后5分钟至1小时之间发现最大杀精效果。
