Morrison B, Shenkin A, McLelland A, Robertson D A, Barrowman M, Graham S, Wuga G, Cunningham K J
Clin Chem. 1979 Oct;25(10):1799-805.
Concentrations of 27 commonly estimated serum constituents were measured in blood sampled from 20 apparently healthy volunteers at 0830, 1230, and 1630 hours on each of four days, at weekly intervals. Time-dependent statistically significant (p less than or equal to 0.01) variation was observed in 12 of the constituents. The 15 constituents that showed no statistically significant diurnal variation included the immunoglobulins, other specific proteins, enzymes, cholesterol, calcium, copper, and magnesium. On the different days of the study consistent temporal patterns were observed in urea, creatinine, phosphate, zinc, bilirubin, triglyceride, total protein, and albumin. The magnitude of variation was particularly great in potassium, iron, zinc, and bilirubin. In general, day-to-day changes in concentration exceeded within-day changes, except for potassium, phosphate, and zinc, for which greater changes could be observed within-day than from one day to another at the same time.
在四周内，每周选取20名表面健康的志愿者，于每天的08:30、12:30和16:30采集血液样本，测定27种常见血清成分的浓度。在12种成分中观察到了随时间变化具有统计学显著性（p≤0.01）的差异。15种未显示出统计学显著昼夜变化的成分包括免疫球蛋白、其他特定蛋白质、酶、胆固醇、钙、铜和镁。在研究的不同日期，尿素、肌酐、磷酸盐、锌、胆红素、甘油三酯、总蛋白和白蛋白呈现出一致的时间模式。钾、铁、锌和胆红素的变化幅度尤为显著。总体而言，除了钾、磷酸盐和锌，浓度的日间变化超过了日内变化，在这三种物质中，同一时间的日内变化比日间变化更大。
