Rose J A, Hoggan M D, Koczot F, Shatkin A J
J Virol. 1968 Oct;2(10):999-1005. doi: 10.1128/JVI.2.10.999-1005.1968.
Adenovirus-associated viruses (AAV) contain double-stranded deoxyribonucleic acid (DNA). DNA from each of the four AAV serotypes was used as template for the in vitro synthesis of complementary (3)H-ribonucleic acids(RNA). An estimation of genetic interrelatedness was made on the basis of hybridization reactions between synthetic AAV RNA and AAV DNA. Heterologous reactions were 27 to 37% of homologous reactions, suggesting that the AAV serotypes are related to about the same extent. AAV-1 synthetic RNA was also reacted with DNA from helper adenovirus types 2, 7, and SV15. Very low levels of RNA binding were observed, but it is not likely that these reactions represent AAV-adenovirus genetic relatedness.
腺相关病毒（AAV）含有双链脱氧核糖核酸（DNA）。四种AAV血清型的每种DNA都被用作体外合成互补（3）H-核糖核酸（RNA）的模板。基于合成的AAV RNA与AAV DNA之间的杂交反应对遗传相关性进行了评估。异源反应是同源反应的27%至37%，这表明AAV血清型的相关性程度大致相同。AAV-1合成RNA也与2型、7型辅助腺病毒和SV15的DNA发生反应。观察到RNA结合水平非常低，但这些反应不太可能代表AAV与腺病毒的遗传相关性。
