Attallah A M, Folks T
Int Arch Allergy Appl Immunol. 1979;60(4):377-82. doi: 10.1159/000232367.
Preincubation of normal human lymphocytes with human interferon for 18-24 h at 37 degrees C resulted in an increase of the activity of both natural killer (NK) cells and antibody-mediated cytotoxic cells (ADCC). The human myeloid line, K-562, which is highly susceptible to NK cells, was employed. ADCC was assessed with antibody-coated chick erythrocytes as targets. NK cells and ADCC were detected in a 4-hour 51Cr release assay. The magnitude of the enhancement was proportionate to the amount of interferon used in preincubation of the effector cells. Preincubation of tumor-target cells with interferon does not increase their susceptibility or resistance to lysis. The major effect of interferon on the cellular metabolism of the tumor-target cell is inhibition of DNA synthesis, but no direct cytotoxic effect was detected. Our findings may be important in understanding the mode of action of interferon in increasing host resistance to a variety of pathogens and tumors. This may be accomplished by inhibiting the growth of the tumor while simultaneously enhancing the natural killing mechanism for immunosurveillance.
将正常人淋巴细胞与人类干扰素在37摄氏度下预孵育18 - 24小时，会导致自然杀伤（NK）细胞和抗体介导的细胞毒性细胞（ADCC）的活性增加。使用了对NK细胞高度敏感的人类髓系细胞系K - 562。以抗体包被的鸡红细胞作为靶标评估ADCC。在4小时的51Cr释放试验中检测NK细胞和ADCC。增强的幅度与在效应细胞预孵育中使用的干扰素量成比例。用干扰素对肿瘤靶细胞进行预孵育不会增加它们对裂解的敏感性或抗性。干扰素对肿瘤靶细胞的细胞代谢的主要作用是抑制DNA合成，但未检测到直接的细胞毒性作用。我们的发现对于理解干扰素在增强宿主对多种病原体和肿瘤的抗性中的作用模式可能很重要。这可能是通过抑制肿瘤生长同时增强免疫监视的自然杀伤机制来实现的。
