虫媒病毒的复制中间体。
Replicative intermediate of an arbovirus.
作者信息
Friedman R M
出版信息
J Virol. 1968 Jun;2(6):547-52. doi: 10.1128/JVI.2.6.547-552.1968.
Abstract
One hour after infection of chick fibroblasts with Semliki Forest virus (SFV), a viral ribonucleic acid (RNA) structure is present which has many of the properties described for the replicative intermediate of several RNA bacteriophages. These properties include a polydisperse nature on sucrose density gradient analysis, ribonuclease resistance, a variation in sedimentation pattern associated with changes in salt concentration, and recovery of infectious viral RNA upon denaturation. Most of the replicative intermediate present in SFV infection appears to be membrane-associated.
摘要
用塞姆利基森林病毒（SFV）感染鸡胚成纤维细胞一小时后，会出现一种病毒核糖核酸（RNA）结构，它具有几种RNA噬菌体复制中间体所描述的许多特性。这些特性包括在蔗糖密度梯度分析中呈多分散性质、对核糖核酸酶有抗性、沉降模式随盐浓度变化而改变，以及变性后可回收感染性病毒RNA。SFV感染中存在的大多数复制中间体似乎与膜相关。
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