5-氟尿嘧啶和6-氮尿嘧啶对卡尔斯伯酵母核糖核酸和蛋白质合成的影响。
Effects of 5-fluorouracil and 6-azauracil on the synthesis of ribonucleic acid and protein in Saccharomyces carlsbergensis.
作者信息
de Kloet S R
出版信息
Biochem J. 1968 Jan;106(1):167-78. doi: 10.1042/bj1060167.
Abstract
- Some effects of 6-azauracil and 5-fluorouracil on protein and RNA synthesis in Saccharomyces carlsbergensis were studied. 2. Both analogues caused a severe inhibition of RNA formation, whereas protein synthesis was much less affected. 3. Induced alpha-glucosidase formation was only slightly impaired. 4. Both analogues caused an inhibition of ribosome formation, although 5-fluorouracil was far more effective. 5. In the presence of the latter analogue abnormal RNA of high molecular weight and of more DNA-like base composition accumulated. On reincubation in medium free of analogue but containing uracil the abnormal RNA disappeared and was replaced by the normally sedimenting high-molecular-weight RNA species.
摘要
- 研究了6-氮尿嘧啶和5-氟尿嘧啶对卡尔斯伯酵母蛋白质和RNA合成的一些影响。2. 两种类似物均导致RNA形成受到严重抑制，而蛋白质合成受影响较小。3. 诱导的α-葡萄糖苷酶形成仅略有受损。4. 两种类似物均导致核糖体形成受到抑制，尽管5-氟尿嘧啶的效果要显著得多。5. 在后者类似物存在的情况下，积累了高分子量且碱基组成更类似DNA的异常RNA。在不含类似物但含有尿嘧啶的培养基中再培养时，异常RNA消失，取而代之的是正常沉降的高分子量RNA种类。
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