Sun G Y, Danopoulos V, Sun A Y
Curr Alcohol. 1979;7:83-91.
Myelin was isolated from the brain stem region of rats which were administered chronically with ethanol either through intragastric tubing (8gm/kg) for 3 weeks or by giving a 15% ethanol ad lib as drink for 1 year. The protein, phospholipid and cholesterol content and the acyl group composition of major phosphoglycerides of myelin were examined. Based on the protein content, there was less myelin isolated from the chronic alcoholic brain samples as compared to controls. Intrinsic changes in membrane components were observed. The phospholipid to protein ratio of myelin was approximately 20% lower in the group subjected chronically to ethanol by drink and 10% lower in the group given ethanol through intubation technique. The cholesterol to protein ratio was also lower in the group given ethanol as drink but the intubated group was not different from controls. Changes in phosphoglyceride acyl group composition were also observed. In general, there was an increase in the proportions of 16:0, 18:0 and 18:1 and a decrease in 20:1, 20:4 and 22:6. The acyl group changes reflected an increase in cellular phospholipase A2 or a decrease in chain desaturation and elongation activity due to chronic ethanol administration. These changes are also reflective of degenerative changes possibly associated with demyelination.
从大鼠脑干区域分离出髓磷脂，这些大鼠通过胃管长期给予乙醇（8克/千克），持续3周，或者给予15%乙醇任其饮用1年。检测了髓磷脂的蛋白质、磷脂和胆固醇含量以及主要磷酸甘油酯的酰基组成。基于蛋白质含量，与对照组相比，从慢性酒精中毒大鼠脑样本中分离出的髓磷脂较少。观察到膜成分的内在变化。长期饮用乙醇组的髓磷脂磷脂与蛋白质的比率比对照组低约20%，通过插管技术给予乙醇组低10%。饮用乙醇组的胆固醇与蛋白质的比率也较低，但插管组与对照组无差异。还观察到磷酸甘油酯酰基组成的变化。一般来说，16:0、18:0和18:1的比例增加，而20:1、20:4和22:6的比例降低。酰基变化反映了由于长期给予乙醇导致细胞磷脂酶A2增加或链去饱和和延长活性降低。这些变化也反映了可能与脱髓鞘相关的退行性变化。
