蛙皮中的N形电流-电位特性。I. 阶跃电压钳制期间的时间变化
The N-shaped current-potential characteristic in frog skin. I. Time development during step voltage clamp.
作者信息
Fishman H M, Macey R I
出版信息
Biophys J. 1969 Feb;9(2):127-39. doi: 10.1016/S0006-3495(69)86374-5.
Abstract
A fast (10 musec) voltage-clamp system similar to that used on nerve axons was applied across the frog skin. An electrical analog is used to obtain the electrical parameters and to estimate the time (300 musec) required to voltage clamp the excitable membrane layer in the skin. The speed of the clamp allows observation of the early development in time of an N-shaped current-potential (I-V) relation. The isochronal I-V curves constructed from step clamp data show the beginning of a negative slope in about 250 musec after successively applied step changes in skin potential (> 200 mv). Subsequently, the negative slope reaches a quasi-steady state interval (0.4-1.5 msec) and then decays and disappears in the next 20 msec. The negative slope I-V characteristic is only found in skins which exhibit spike generation under current clamp.
摘要
一种类似于用于神经轴突的快速（10微秒）电压钳系统被应用于蛙皮。使用一个电模拟装置来获取电参数，并估计对皮肤中可兴奋膜层进行电压钳制所需的时间（300微秒）。钳制的速度使得能够观察到N形电流 - 电位（I - V）关系在时间上的早期发展。从阶跃钳数据构建的等时I - V曲线显示，在皮肤电位相继施加阶跃变化（> 200毫伏）后约250微秒开始出现负斜率。随后，负斜率达到一个准稳态区间（0.4 - 1.5毫秒），然后在接下来的20毫秒内衰减并消失。负斜率I - V特性仅在电流钳制下表现出动作电位产生的皮肤中发现。
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