Gershon H, Feldman M
Immunology. 1969 Feb;16(2):175-85.
Using a method for cloning lymph node (LN) derived cells in the spleens of lethally irradiated mice, it was determined that the macroscopic colonies observed were haemopoietic whereas the intercolonial cells were primarily lymphoid. The immune response detected in isolated colonies was shown to be attributable to contamination of the clones by immunologically reactive interclonal cells. It was also demonstrated that pre-treatment of LN donor mice with PHA enhanced cloning efficiency but was not a necessary procedure for obtaining LN-derived colonies in the spleens of irradiated recipient mice.
利用一种在致死性照射小鼠脾脏中克隆淋巴结（LN）来源细胞的方法，确定观察到的宏观集落是造血性的，而集落间细胞主要是淋巴细胞。在分离的集落中检测到的免疫反应被证明是由于具有免疫反应性的集落间细胞对克隆的污染所致。还证明，用PHA对LN供体小鼠进行预处理可提高克隆效率，但对于在受照射受体小鼠脾脏中获得LN来源的集落而言，这不是必需的步骤。
