Bick P H, Carpenter A B, Holdeman L V, Miller G A, Ranney R R, Palcanis K G, Tew J G
Infect Immun. 1981 Oct;34(1):43-9. doi: 10.1128/iai.34.1.43-49.1981.
The objective of this research was to determine whether gram-negative bacteria frequently isolated from periodontally diseased sites contained polyclonal B-cell activators. Polyclonal B-cell activation, which results in nonspecific activation of multiple B-cell clones was analyzed by a hemolysis-in-gel assay designed to detect a broad range of antibody specificities. Extracts from numerous bacterial strains, including Bacteroides gingivalis, Bacteroides melaninogenicus subsp. melaninogenicus, B. melaninogenicus subsp. intermedius, Fusobacterium nucleatum, Selenomonas sputigena, Capnocytophaga ochracea, and Actinobacillus actinomycetemcomitans, were tested. Extracts of the above organisms were found to stimulate polyclonal antibody responses in cultures of normal human peripheral blood lymphocytes, although the magnitude of stimulation varied among the extracts. Optimal antibody-forming cell responses were found at stimulator doses between 5 and 1,000 micrograms/ml. We conclude that the resident gram-negative subgingival flora associated with periodontal lesions possesses potent polyclonal B-cell activators. These activators may contribute to disease pathogenesis by inducing B lymphocytes to produce antibody, osteolytic factors, or both and possibly other mediators of inflammation.
本研究的目的是确定从牙周病损部位频繁分离出的革兰氏阴性菌是否含有多克隆B细胞激活剂。通过旨在检测广泛抗体特异性的凝胶溶血试验分析了导致多个B细胞克隆非特异性激活的多克隆B细胞激活情况。测试了多种细菌菌株的提取物，包括牙龈拟杆菌、产黑色素拟杆菌黑色素亚种、产黑色素拟杆菌中间亚种、具核梭杆菌、龈沟栖硒单胞菌、赭色二氧化碳嗜纤维菌和伴放线放线杆菌。发现上述生物体的提取物可刺激正常人外周血淋巴细胞培养物中的多克隆抗体反应，尽管不同提取物的刺激程度有所不同。在刺激剂剂量为5至1000微克/毫升之间时发现了最佳抗体形成细胞反应。我们得出结论，与牙周病变相关的龈下革兰氏阴性常驻菌群具有强大的多克隆B细胞激活剂。这些激活剂可能通过诱导B淋巴细胞产生抗体、溶骨因子或两者以及可能的其他炎症介质而导致疾病发病机制。
