El Kheir A A, Ahmed A K, El Bardicy G Z, Amer M M
Arzneimittelforschung. 1977;27(3):551-5.
A semimicro-titrimetric method for the determination of heparin is developed. The method depends upon the determination of the sulphate content of the acid drolysate of heparin by adding an excess of barium chloride and back titration with standard sulphuric acid, using sodium rhodizonate as indicator. The method measures the biological activity of 10-50 mg of heparin, with a mean accuracy (p = 0.05) 99.73 +/-0.81%. Application of the proposed method for the determination of heparin in some pharmaceutical preparations is carried out by precipitation of the intact heparin molecule with cetyltrimethylammonium bromide, ashing of the precipitate and determination of its sulphate content; alkaline and acid degradation products do not interfere with the proposed procedure.
开发了一种用于测定肝素的半微量滴定法。该方法基于通过加入过量的氯化钡并以标准硫酸进行返滴定，以玫红酸钠为指示剂，测定肝素酸水解产物中的硫酸盐含量。该方法可测定10 - 50mg肝素的生物活性，平均准确度（p = 0.05）为99.73±0.81%。通过用十六烷基三甲基溴化铵沉淀完整的肝素分子、将沉淀灰化并测定其硫酸盐含量，将所提出的方法应用于一些药物制剂中肝素的测定；碱性和酸性降解产物不干扰所提出的程序。
