Olsson P, Lagergren H, Larsson R, Rådegran K
Thromb Haemost. 1977 Apr 30;37(2):274-82.
A stable heparinized surface was prepared by sequential treatment of polyethylene with water solutions of hexadecylamine hydrochloride, heparin and glutardialdehyde. In order to explain the "non-thrombogenic" properties of this surface, it was evaluated with regard to prevention of platelet adhesion and aggregation. Human heparinized blood (2 and 10 IU/ml) with 51Cr-labelled autologous platelets was rotated for 60 minutes in untreated and heparin-treated circular tubings. The surface area/blood volume ratio was varied and an air-blood interface was present. In untreated tubings, platelet adhesion and aggregation increased in proportion to the size of the surface area/blood volume ratio, irrespective of the heparin concentrations of the blood. In the heparin-treated tubings, there was no measurable platelet adhesion to the surface and no platelet aggregation in the blood. The difference between the heparinized and the untreated surfaces with regard to platelet adhesion was discernible even after 10 minutes storage of stagnant blood. It is concluded that platelet adhesion and aggregation induced by exposure of blood to a foreign surface in an in vitro experimental model can be prevented by a stable heparin coating of the surface.
通过用十六烷基胺盐酸盐、肝素和戊二醛的水溶液依次处理聚乙烯，制备了一种稳定的肝素化表面。为了解释该表面的“抗血栓形成”特性，对其预防血小板粘附和聚集的能力进行了评估。将含有51Cr标记的自体血小板的人肝素化血液（2和10 IU/ml）在未处理和肝素处理过的圆形管中旋转60分钟。表面积/血容量比有所变化，并且存在气-血界面。在未处理的管中，无论血液中的肝素浓度如何，血小板粘附和聚集均与表面积/血容量比的大小成比例增加。在肝素处理过的管中，未检测到血小板与表面的粘附，血液中也没有血小板聚集。即使在静态血液储存10分钟后，肝素化表面和未处理表面在血小板粘附方面的差异也是明显的。得出的结论是，在体外实验模型中，通过表面的稳定肝素涂层可以防止血液暴露于异物表面所诱导的血小板粘附和聚集。
