Larsson R L, Hjelte M B, Eriksson J C, Lagergren H R, Olsson P
Thromb Haemost. 1977 Apr 30;37(2):262-73.
Heparin can be bound to polymer surfaces by precipitation of an ionic heparin-amine complex which can be stabilized against dissolution by treatment with glutardialdehyde. The aim of this investigation was to study the degree and course of desorption of heparin from such a heparinized surface on contact with blood. This desorption must be considered when analysing the interaction between the heparinized surface and blood. Different heparinized surfaces were prepared by using 35S-labelled heparin, and the desorption of heparin on exposure in vitro to citrated plasma or heparinized blood as well as during exposure at in vivo conditions was quantified. During in vitro experiments, the glutardialdehyde stabilized surface became stable with no further desorption of heparin after an initial loss of about 3% of the initial surface-bound heparin. Under in vivo conditions, there was an initial loss of about 12%. There was no further loss from surfaces inserted into the circulation of the dog for seven days as compared to those inserted for one hour.
肝素可通过离子型肝素 - 胺复合物的沉淀结合到聚合物表面，该复合物可用戊二醛处理以防止溶解而稳定下来。本研究的目的是研究肝素从这种肝素化表面与血液接触时的解吸程度和过程。在分析肝素化表面与血液之间的相互作用时，必须考虑这种解吸。使用35S标记的肝素制备了不同的肝素化表面，并对体外暴露于枸橼酸盐血浆或肝素化血液以及体内暴露期间肝素的解吸进行了定量。在体外实验中，戊二醛稳定的表面在最初损失约3%的初始表面结合肝素后变得稳定，没有进一步的肝素解吸。在体内条件下，最初损失约12%。与插入一小时的表面相比，插入狗循环系统七天的表面没有进一步的损失。
