使用内因子、57钴维生素B12和包被活性炭测定血清中维生素B12的改进方法
Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.
作者信息
Raven J L, Robson M B, Walker P L, Barkhan P
出版信息
J Clin Pathol. 1969 Mar;22(2):205-11. doi: 10.1136/jcp.22.2.205.
Abstract
An improved and simplified method is described for the measurement of vitamin B(12) in serum using intrinsic factor, (57)CoB(12), and coated charcoal. The extraction of serum in the presence of cyanide and the incorporation of B(12)-deficient serum into the intrinsic factor control has increased the accuracy of the method for both sera and crystalline B(12) solutions. There are interesting differences between the results obtained for some sera by the isotope and L. leichmannii methods and the reasons for these differences are discussed.
摘要
描述了一种改进且简化的方法，用于使用内因子、（57）CoB12和包被活性炭测定血清中的维生素B12。在氰化物存在下对血清进行提取以及将缺乏B12的血清纳入内因子对照，提高了该方法对血清和结晶B12溶液的准确性。讨论了通过同位素法和莱氏乳酸杆菌法对某些血清获得的结果之间存在的有趣差异以及这些差异的原因。
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