使用内因子、57钴维生素B12和包被活性炭测定血清中维生素B12的改进方法
Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal.
作者信息
Raven J L, Robson M B, Walker P L, Barkhan P
出版信息
J Clin Pathol. 1969 Mar;22(2):205-11. doi: 10.1136/jcp.22.2.205.
Abstract
An improved and simplified method is described for the measurement of vitamin B(12) in serum using intrinsic factor, (57)CoB(12), and coated charcoal. The extraction of serum in the presence of cyanide and the incorporation of B(12)-deficient serum into the intrinsic factor control has increased the accuracy of the method for both sera and crystalline B(12) solutions. There are interesting differences between the results obtained for some sera by the isotope and L. leichmannii methods and the reasons for these differences are discussed.
摘要
描述了一种改进且简化的方法，用于使用内因子、（57）CoB12和包被活性炭测定血清中的维生素B12。在氰化物存在下对血清进行提取以及将缺乏B12的血清纳入内因子对照，提高了该方法对血清和结晶B12溶液的准确性。讨论了通过同位素法和莱氏乳酸杆菌法对某些血清获得的结果之间存在的有趣差异以及这些差异的原因。
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本文引用的文献
1
2
Isotopic determination of vitamin B12 binding capacity and concentration.维生素B₁₂结合能力和浓度的同位素测定
Proc Soc Exp Biol Med. 1962 Mar;109:604-8. doi: 10.3181/00379727-109-27282.
3
Assay of vitamin B12 in blood. A simple method.血液中维生素B12的测定。一种简单方法。
Lancet. 1961 Jul 1;2(7192):25-6. doi: 10.1016/s0140-6736(61)92711-8.
4
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Clin Sci. 1955 Nov;14(4):661-7.
5
The determination of vitamin B12 activity in human serum.人血清中维生素B12活性的测定
J Biol Chem. 1952 Nov;199(1):433-42.
6
Radioisotopic assay of serum vitamin B 12 with the use of DEAE cellulose.使用二乙氨基乙基纤维素进行血清维生素B₁₂的放射性同位素测定。
J Lab Clin Med. 1966 Sep;68(3):510-22.
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Results with radioisotopic assay of serum B12 using serum binding agent.采用血清结合剂对血清维生素B12进行放射性同位素测定的结果。
J Clin Pathol. 1967 Sep;20(5):683-6. doi: 10.1136/jcp.20.5.683.
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Comparison of the radioisotope dilution-coated charcoal method and a microbiological method (L. leichmannii) for measuring vitamin B12 in serum.放射性同位素稀释包被活性炭法与微生物法（莱氏乳杆菌法）测定血清中维生素B12的比较。
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