血清维生素B12检测的实验室间比对
Interlaboratory comparison of serum vitamin B12 assay.
作者信息
Mollin D L, Hoffbrand A V, Ward P G, Lewis S M
出版信息
J Clin Pathol. 1980 Mar;33(3):243-8. doi: 10.1136/jcp.33.3.243.
Abstract
The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in reference laboratories by microbiological assay with both E. gracilis and L. leichmannii. In general, RID gave higher results than microbiological assay. The need for suitable reference sera is emphasised.
摘要
英国全国性实验室间试验的参与者对血清维生素B12进行微生物学检测和放射性同位素稀释（RID）检测的结果已进行了比较。各个参与者之间存在很大差异，在莱氏乳杆菌微生物学检测和RID方法中尤为明显，而在参考实验室中，使用纤细裸藻和莱氏乳杆菌进行微生物学检测时，获得了极佳的相关性、重现性和回收率。一般来说，RID检测结果高于微生物学检测。强调了需要合适的参考血清。
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