Reháćek J, Beran K, Bićík V
Appl Microbiol. 1969 Mar;17(3):462-6. doi: 10.1128/am.17.3.462-466.1969.
Microbial cells were disintegrated in a new type of rotary disintegrator with a disc stirrer by a combination of shear force layers, collisions, and rolling of glass beads which were brought into motion by the stirrer. The rate of disintegration at a given dry bed volume of Ballotini beads and a given volume of cell suspension is proportional to the peripheral velocity of the stirrer up to 18 m/sec. Horizontal arrangement of the stirrer increases the effectiveness about five times; 100% disintegration of yeast cells was achieved under optimal conditions within 72 sec at a concentration of 3.5g (dry weight)/100 ml of suspension, and within 96 sec at a concentration of 10.5g (dry weight)/100ml. At 17.5 g (dry weight)/100 ml, the stirrer began to slip. The cell walls of yeast were obtained at the desired degree of crushing and the course of purification was determined by infrared spectral analysis.
微生物细胞在一种新型的带有圆盘搅拌器的旋转式粉碎机中被破碎，通过搅拌器带动玻璃珠运动产生的剪切力层、碰撞和滚动的组合来实现。在给定的巴洛替尼珠干床体积和给定的细胞悬浮液体积下，破碎速率与搅拌器的圆周速度成正比，最高可达18米/秒。搅拌器的水平布置使效率提高约五倍；在最佳条件下，酵母细胞在浓度为3.5克（干重）/100毫升悬浮液时，72秒内实现100%破碎，在浓度为10.5克（干重）/100毫升时，96秒内实现100%破碎。在17.5克（干重）/100毫升时，搅拌器开始打滑。通过红外光谱分析确定了酵母细胞壁达到所需破碎程度以及纯化过程。
