人甲胎蛋白的纯化与放射免疫测定：聚集体对放射免疫测定的影响
Purification and radioimmunoassay of human alpha-1-fetoprotein: the effect of aggregates on the radioimmunoassay.
作者信息
Young J L, Reid R G, Crawford J W
出版信息
Clin Chim Acta. 1976 May 17;69(1):11-20. doi: 10.1016/0009-8981(76)90466-6.
A simple method for the extraction of alpha1-fetoprotein from fetal serum in high yield and of sufficient purity for use as radio-iodinated tracer in a radioimmunoassay is described. The purified alpha1-fetoprotein formed aggregates during storage. The presence of 125I-labelled aggregates affected the double antibody radioimmunoassay by increasing the non-specific bound, resulting in decreases assay sensitivity. Freshly prepared 125I-labelled monomer alpha1-fetoprotein is prepared for each assay. The radioimmunoassay described is sensitive to between 100 and 200 pg per tube. Normal adult serum levels were found to be in the range from less than 1 mug/l to 16 mug/l.
本文描述了一种从胎血中高产率提取甲胎蛋白的简单方法，所提取的甲胎蛋白纯度足以用作放射免疫分析中的放射性碘化示踪剂。纯化的甲胎蛋白在储存过程中会形成聚集体。125I标记的聚集体的存在会增加非特异性结合，从而影响双抗体放射免疫分析，导致分析灵敏度降低。每次分析都要制备新鲜的125I标记的单体甲胎蛋白。所述放射免疫分析每管对100至200皮克敏感。发现正常成人血清水平在每升低于1微克至16微克的范围内。
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