Dell H D, Fiedler J, Wäsche B
Arzneimittelforschung. 1977;27(6B):1312-6.
The determination of 2-(2-hydroxyethoxy)-ethyl-N-(a,a,a-trifluoro-m-tolyl)-anthranilate (etofenamate, active principle of Rheumon gel) following its isolation from biological material is reported. Depending on the method of extraction etofenamate, free and alkali-labile conjugated flufenamic acid, total conjugates or the sum of CF3-containing compounds (sum of metabolites) are isolated. Separation is achieved by TLC, quantitative determination is made by degradation to flufenamic acid and fluorimetric measurement in CCl4/trichloracetic acid at 372/445 nm. Etofenamate can be identified by TLC, derivatisation, UV- and fluorescence spectroscopy and differentiated from its metabolites. It is demonstrated that etofenamate is the main component of fenamates in inflamed tissue.
报道了从生物材料中分离出2-(2-羟基乙氧基)乙基-N-(α,α,α-三氟间甲苯基)邻氨基苯甲酸酯（依托芬那酯，风湿凝胶的活性成分）后的测定方法。根据提取方法，可分离出依托芬那酯、游离的和对碱不稳定的共轭氟芬那酸、总共轭物或含CF3化合物的总和（代谢物总和）。通过薄层色谱法进行分离，通过降解为氟芬那酸并在372/445nm波长下于四氯化碳/三氯乙酸中进行荧光测定来进行定量测定。依托芬那酯可通过薄层色谱法、衍生化、紫外和荧光光谱法进行鉴定，并与其代谢物区分开来。结果表明，依托芬那酯是炎症组织中芬那酸盐的主要成分。
